Microbial Interactions at Nanobiotechnology Interfaces by R. Navanietha Krishnaraj & Rajesh K. Sani

Author:R. Navanietha Krishnaraj & Rajesh K. Sani [Krishnaraj, R. Navanietha & Sani, Rajesh K.]
Language: eng
Format: epub
ISBN: 9781119617174
Published: 2021-10-07T00:00:00+00:00

6.8.3 Effect of Surrounding Environment

6.8.3.1 Effect of Media Composition on Corona Formation

At in vitro levels, protein corona formation and cellular uptake are studied in culturing the cells in RMPI or DMEM medium supplemented with fetal bovine serum albumin. As the cell grows at the expense of cell culture medium, the concentration of the protein always varies distinctly based on the growth rate of cells. Therefore, the abundance of proteins differs significantly. This is one of the variables that affect the protein corona formation (Albanese et al., 2014). Maiorano et al. (2010) studied the impact of media composition on the evolution of protein corona on gold nanoparticles capped with citrate. The studies confirmed that the kinetics of corona evolution was low in RPMI medium than with DMEM medium. Cellular uptake of gold nanoparticles by HeLa cells (human cervical cancer cell lines) and U937 (human leukemic monocyte lymphoma cells) cultured in RPMI medium was high compared to RPMI medium. The concentration of plasma or serum used in the culture directly influences the hydrodynamic size of the protein corona. This is confirmed with silica nanoparticles and carboxylated polystyrene nanoparticles. Binding of complement and immunoglobulins onto charged nanoparticles increases their elimination through opsonization (Roser, Fischer, & Kissel, 1998).

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